METHODS AND MATERIALS FOR THE GROWTH OF PRIMATE-DERIVED PRIMORDIAL STEM CELLS IN FEEDER-FREE CULTURE

摘要

Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primatederived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.