METHOD AND KIT FOR DETERMINING SEVERE INFLAMMATORY REACTIONS TO MOSQUITO BITES

摘要

Mosquito extract consisting essentially of antigens solely to allergens inmosquito saliva. Isolated and purified recombinant mosquito saliva antigensfor use in skin tests, immunoassays and immunotherapy for allergic reactionsto mosquito bites. A method for skin testing and determining the undertakingof immunotherapy includes the steps of administrating the isolated andpurified mosquito salivary antigens to a patient and then recognizing skinreaction thereto is a positive indication of needing therapy. A kit and animmunoassay are also provided for measurement of mosquito salivary allergenspecific IgE and IgG wherein the assay includes the isolated and purifiedrecombinant mosquito allergens as a substrate to which allergen specific IgEand IgG binds. Antibodies directed against the recombinant salivary allergensof mosquitoes are also disclosed.