Detection and typing of the (icea) gene of (helicobacter pylori)

摘要

The present invention relates to a method for detection and/or typing of alleles of the iceA gene of H. pylori present in a biological sample, with said method comprising: i) amplifying polynucleic acids of the iceA gene by use of primers that permit specific amplification either of a fragment of iceA1 variants or of a fragment of iceA2 variants, and/or ii) hybridizing polynucleic acids of the iceA gene, possibly after amplification as in step i), with at least one probe that specifically hybridizes to either iceA1 variants or to iceA2 variants. In order to enable this method, the present invention discloses nucleic acid sequences of iceA1 and iceA2 variants. Primers and probes that can be used to carry out said method are also disclosed. The present invention further relates to diagnostic kits that allow to perform said method. IceA1 and IceA2 protein sequences are also disclosed, as well as antibodies directed against these sequences.