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Assaying of antibodies directed against one or more antigens of (helicobacter pylori) in biological liquids by heterogeneous immunologic method of the reversetype
Assaying of antibodies directed against one or more antigens of (helicobacter pylori) in biological liquids by heterogeneous immunologic method of the reversetype
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机译:通过反向异型免疫学方法测定针对生物液体中一种或多种幽门螺杆菌抗原的抗体
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摘要
Method for the determination of the presence or absence of immunoglobulins directed against one or more Helicobacter pylori antigens, in animal biological liquids, which is carried out using an immunobiologic heterogeneous competition test commonly referred to as "reverse" test. The presence or absence of specific immunocomplexes is assessed either in a direct fashion, which is when the reaction signal is directly carried by the antigen or the antigens of Helicobacter pylori, or in an indirect fashion, which is when the immunologic reaction signal is obtained by adding one or more tracing substances that bind to the antigen or to the antigens of Helicobacter pylori that were previously captured by the immunoglobulins. By comparision with the methods currently used in the diagnostic field, this method has the following main features: a) use of undiluted biological liquid in the diagnostic test, because the test is of the competitive type with respect to the solid phase used; b) determination of the presence of antibodies directed against one or more antigens of Helicobacter pylori in biological liquids, by a quantitative assessment of the antigens bonded to the solid phase; c) use of standard solutions made up of mixtures containing variable concentrations of anti-Helicobacter pylori immunoglobulins and immunoglobulins that do not react with the antigens used in the assay.
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