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Use of molecular markers for genotype determination of the ogura rf gene in brassica napus

机译:分子标记在甘蓝型油菜中ogura rf基因的基因型测定中的应用

摘要

This invention relates to Brassica plants and methods of breeding Brassica plants. More particularly, the invention relates to the use of molecular markers for determining the genotype of the Ogura Rf gene in Brassica plants at an early stage in their development. The method involves using a combination of primers derived from RAPD markers in PCR reactions to allow discrimination between homozygous, heterozygous and non-restorer or sterile genotypes. Also disclosed are combinations of primers which allow such discrimination.
机译:本发明涉及芸苔属植物和芸苔属植物的育种方法。更具体地,本发明涉及分子标记物在芸苔属植物发育的早期阶段用于确定芸苔属植物中Ogura Rf基因的基因型的用途。该方法包括在PCR反应中使用源自RAPD标记的引物的组合,以区分纯合,杂合和非还原或无菌基因型。还公开了允许这种区分的引物的组合。

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