NUCLEAR TRANSFER FOR PRODUCTION OF TRANSGENIC ANIMAL EMBRYO

摘要

A method of generating a transgenic animal embryo which method includes providing a donor nucleus and a recipient cell; removing the nucleus for the recipient cell; introducing the donor nucleus into the enucleated cell to produce a couplet; and maintaining the couplet in a suitable medium for a period sufficient to allow the cell to recover a substantially normal shape. The method can be applied for porcine embryos and is exemplified with heterogenic expression of α1,3-galactosyl transferase.