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INSIDE-OUT CROSS-LINKED AND COMMERCIAL-SCALE HYDROGELS, AND SUB-MACROMOLECULAR SELECTIVE PURIFICATION USING THE HYDROGELS
INSIDE-OUT CROSS-LINKED AND COMMERCIAL-SCALE HYDROGELS, AND SUB-MACROMOLECULAR SELECTIVE PURIFICATION USING THE HYDROGELS
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机译:内联交联和商业规模的水合物,以及使用水合物进行的大分子选择性纯化
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摘要
Relating to chromatographic processes and ion-exchange and affinity matrices, a spatial installation method for a bifunctional reagent that cross-links and/or activates a polymer matrix is disclosed, with inside-outside installation of a bifunctional reagent on and within a polymer matrix. The polymer matrix is cellulose, agarose, or chitosan particles. The installation may be followed by inside-outside ligand attachment, by further reacting the matrix with a ligand or ionic group so that a higher concentration of ligand or ionic moiety occurs on the intra-particle volume than on the outer matrix surface.
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