DETECTION OF MICROSPORIDIA AND MICROSPORIDIAN INFECTIONS

摘要

mikrosporidien are an unusual group of parasites is difficult to prove. the new detection method, the detection of mikrosporidien without prior cultivation of the species without dna amplifizierungstechniken and without microscopic methods possible.$a mrna a culture of encephalitozoon cuniculi a cdna bank and immunogenic clones produced antibodies against e. cuniculi received to identify and umklonierung into an expression vector and recombinant antigens expressed.antigens further, not kultivierbarer mikrosporidienarten at dna level by hybridization homologer genabschnitte and subsequent isolation and expression is generated.the antigens in large quantities and purity available, either directly for the serological detection of antibodies or for immunization and for the production of mono - and received antibodies can be used.with these antibodies, which can mikrosporidien directly by detection of antigens in the clinical material of humans and animals, as well as from environmental samples and foodstuffs to be shown without prior cultivation of the pathogen dna amplifizierungstechniken or mikr oskopische methods are necessary.$a indication is the presence of mikrosporidien and mikrosporidieninfektionen, especially from clinical material of humans and animals, as well as from environmental samples and food.