NUCLEOTIDE PROBES AND METHOD FOR DETERMINING HLA DQB1 TYPING

摘要

Nucleotide probe selected in particular among the following: TG uCGT TAT GTG ACC AGA/u, GTuG CGT CTT GTG ACC AGA/u, GT uCTT GTA ACC AGA CAC/u AT, CGuT CTT GTA ACC AGA TAC/u AT, CGuT CTT GTG AGC AGA AGC/u AT, CG uACC GAG CTC GTG CGG CG/uT G, G TuAC CGG GCA GTG AC/uG C, G AuCG CCG CTG GGI CCG CCT G/u, G AuCG CCG CTG GGG CCG CCT G/u, G GuAG GGI ACC CIG GCI GA/uG T, G GuAG GGG ACC CGG GCG GA/uG T, TCuG GTG GAC ACC GTA TGC AG/uA C, GG uACG GAG CGC GTG /uCG, C uATC TAT AAC CGA GA/u, and their complementary sequences, being understood that the said probes have at least the underlined sequence, and can further contain one or two bases selected, respecting the sequence continuity, among the non-underlined bases. These probes are useful for determining a person's HLA DQ beta genotype. They have in particular the advantage of being useable at a single hybridization temperature, in particular 37 DEG C.