Recombinant thermostable tryptophanases A and B, a process for their preparation and a process for the preparation of L-tryptophan using them

摘要

The present invention relates to a recombinant thermostable tryptophanase A and B, a process for producing the same, and a process for producing L-tryptophan using the same. More particularly, the present invention relates to a process for producing L-tryptophan from thermophilic microorganisms producing heat resistant tryptophanase Isolating genes encoding A and B to determine its nucleotide sequence, preparing recombinant plasmids containing the heat resistant tryptophanase genes, transforming Escherichia coli, and culturing the transformed strains, Topanase A and B can be genetically engineered. Using the recombinant tryptophanase thus produced, L-tryptophan, a precursor of neurotransmitter from pyruvic acid and indole, can be produced inexpensively with high yield.