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BIOLOGICALLY ACTIVE FRAGMENT OF BACILLUS STEAROTHERMOPHILUS DNA POLYMERASE
BIOLOGICALLY ACTIVE FRAGMENT OF BACILLUS STEAROTHERMOPHILUS DNA POLYMERASE
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机译:嗜热芽孢杆菌DNA聚合酶的生物活性片段
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摘要
The present invention is directed to an isolated and purified DNA encoding a biologically active fragment of a thermostable, full length DNA polymerase I enzyme of Bacillus stearothermophilus. More particularly, the invention is directed to a DNA encoding an approximately 66,000 dalton DNA polymerase that lacks 273 amino acids from the N-terminus of the approximately 96,000 dalton B. stearothermophilus DNA polymerase I, and to the protein encoded thereby which has been designated the B. stearothermophilus DNA polymerase I exo- fragment. The enzyme fragments are useful in DNA sequencing, cDNA preparations, thermophilic Strand Displacement Amplification and other molecular biology applications.
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