SYNTHETIC BACILLUS THURINGIENSIS GENE ENCODING CRYLCA (CRYLC) TOXIN

摘要

A method of gene synthesis is disclosed. The gene synthesis method permits the codons of [m] natural gene to be changed to allow preferential transcription and translation of the synthetic gene in transgenic organisms. The method utilizes a combination of enzymatic and chemical synthesis of DNA and significantly reduces the cost, time and number of steps required for construction of synthetic genes.