Recombinant heat-resistant D-amino acid aminotransferase and method for producing D-amino acid using the same

摘要

The present invention is based on the finding that the nucleotide sequence of the thermostable D-amino acid aminotransferase gene is determined after cloning a gene encoding a thermostable D-amino acid aminotransferase from the thermophilic microorganism Bacillus sp. KLS-01 isolated from soil . In addition, the present invention relates to a method for transforming Escherichia coli XL1Blue with a recombinant plasmid pHKLS01 containing a recombinant heat-resistant D-amino acid aminotransferase gene, culturing the transformant XL1Blue / pHKLS01 (KCTC 0394BP) Amino acid production process characterized in that it is used as a biocatalyst in a biocatalytic conversion process for producing D-amino acid from -keto acid after producing lyase.