DszD utilization in desulfurization of DBT by rhodococcus sp.IGTS8

摘要

Present invention relate to the discovery that increasing desulfuration of fossil fuels reaction speed addition oxidoreducing enzyme and biocatalyst. The invention is a kind of method close to rate of sulphur expulsion for improving the fossil fuel containing organic sulfur compound, include the following steps: one kind) fossil fuel is contacted with containing the oxidoreducing enzyme that can crack carbon-sulfide linkage biocatalyst and a speedup amount, the mixture of fossil fuel and water phase is formed whereby; Step b) be broken under the conditions of the mixture of step a) is maintained at enough the organosulfur molecules of carbon-sulfur bond by biocatalyst, and then obtain the fossil fuel of organic sulfur content reduction; C) fossil fuel of organic sulfur content reduction is separated from resulting water phase. Furthermore the invention further relates to the recombinant microorganism containing one or more recombinant DNA molecules, which coding can encode the biocatalyst of the desulfurization of fossil fuel containing organosulfur molecules and oxidoreducing enzyme. The present invention also relates to compositions, and it includes (a) can be by the biocatalyst b oxidoreducing enzyme of the desulfurization of fossil fuel containing organosulfur molecules.