A method for confirming the attenuation of a live-attenuated vaccine based on a 45-hPi-iV-3 strain and the vaccine in the vaccine

摘要

The present invention is based on the correlation between two attenuating disorders of the cp45 strain and specific defects in the cp45 genome. Specifically, significant attenuation of cp45 due to temperature sensitivity and low temperature adaptive phenotype is associated with the mutation of the L gene of cp45. Secondary mutations in the gene encoding erythrocyte aggregate-neuraminidase (HN) in the cp45 strain were also found to be another attenuation mutation. The mutual relationship between the attenuating disorders of the two cp45s and specific genes allows several practical applications. It is now possible to combine the mutated L and / or HN genes of cp45 into the target viral genome or, alternatively, to express other viral surface antigen genes in cp45, to produce other wild-type HPIV-3 and HPIV-An attenuated vaccine may be produced.