REPRODUCTION METHOD OF REGENERATED GINSENG EXPLANTET FROM SOMATIC EMBRYO CULTURE

摘要

The present invention was inoculated into a solid culture medium in which the oocytes and cytokinin were added to MS culture medium in which nitrogen concentration was modified, and the cotyledon embryos grown from cotyledons extracted from ginseng seeds were grown at 4-25 ° C. Process of growing, inoculating the grown cotyledon embryo into a solid incubator with oxine and cytokinin added to MS medium modified nitrogen concentration, and incubating at 4-25 ℃ for 2-6 weeks to mature cotyledon embryo It consists of ginseng plant manufacturing method comprising the step of inoculating cotyledon-type embryos in a solid incubator with gibberellin to the MS medium modified nitrogen concentration and incubating at 4-25 ℃. According to the present invention, since the globular embryos which have grown somatic embryos of ginseng are developed into cotyledon embryos and undergo a sufficient maturation process, ginseng plants with the same stem and root as the parent plants are obtained.