The yeast extract from the substrate yeast of 10% (v / v) concentration was produced using the yeast cell wall lyase produced by the strain D. YCH-37 (Dicyma sp. YCH-37) KFCC-10851. The substrate yeast of the logarithmic growth stage was heat-treated at 60-80 ° C. for 2-4 hours before the reaction, and then using 40-60 units of enzyme for 20-150 ° C. at 20-50 ° C. and pH 5.0-9.0. Reacted. Autolysis of substrate yeast was reacted for 20-30 hours at 40-70 ° C. and pH 5.0-7.0 by adding 5.0% (v / v) ethyl acetate as a reaction accelerator. As a result of comparing the amount of yeast extract obtained from the enzymatic reaction with the amount of yeast extract obtained from the autolyse, the relative yield of the yeast extract finally obtained by the enzymatic reaction was 62.1%, which was higher in yield than the yeast extract obtained from the autolyse. A three-fold increase and a time-shortening effect of about 60 times were obtained.
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