THE METHOD OF CULTURING LIQUID STARTER OF BASIDOMYCETIS AND LIQUID STARTER

摘要

The present invention relates to a liquid spawn cultivation method of basidiomycetes and a liquid spawn thereof, and more particularly to a method capable of producing a large amount of liquid spawn useful for the cultivation of various mushrooms of high quality and a liquid spawn obtained by the method.;The present invention is a liquid culture method of common basidiomycetes, the pre-culture step (a) 2 ~ 7% (w / v) carbon source, 0.1 ~ 1.0% (w / v) nitrogen source, KH ₂ PO ₄ 0 ~ 0.15 Inoculate the plated basidiomycetes in the preculture consisting of% (w / v), MgSO ₄ · 7H ₂ O 0-0.15% (w / v), and the remaining distilled water, and (b) the inoculated preculture Incubated at 80 ~ 200 rpm, 22 ~ 30 ℃ shaking culture in 6 to 8 days; The above liquid culture process was carried out by (a) carbon source 2-7% (w / v), nitrogen source 0.1-1.0% (w / v), KH ₂ PO ₄ 0-0.15% (w / v), MgSO ₄ 7H ₂ O 0 ~ 0.15% (w / v), and prepare a liquid culture consisting of the remaining distilled water, (b) aseptic auto-inoculation of the pre-inoculation source to the liquid culture solution, (c) the inoculated The liquid culture consists of aeration of the culture medium at 22-28 ° C. with sterilized air on the order of 0.1-1.0vvm for 5-12 days.;The present invention is characterized in that it is composed of a series of steps which are inseparably connected to the preculture process and the liquid culture process under stringent conditions, respectively, after collecting the prokaryote of basidiomycetes.