Identification of predominant lactic acid bacteria and yeasts of Turkish sourdoughs and selection of starter cultures for liquid sourdough production using different flours and dough yields

摘要

Eight samples of mature sourdough were collected from fi ve provinces of Turkey. Lactic acid bacteria and yeasts were isolated and identifi edudand used in different combinations to produce liquid sourdoughs. Microbiological and physicochemical characteristics of the experimental sourdoughsudmade with different fl our types and dough yields were studied. The main lactic acid bacteria species identifi ed were Lactobacillus (L.) sanfranciscensis,udPediococcus pentosaceus, L. plantarum, L. namurencis, L. rossiae, Leuconostoc mesenteroides and L. zymae. L. spicheri, L. paralimentarius, L. mindensis,udL. farciminis, L. acetotolerans, L. casei, Enterococcus faecium and Enterococcus durans were also found in sourdoughs at subdominant levels. Amongudyeasts, mainly Saccharomyces cerevisiae, but also Pichia guiliermondii and Torulaspora delbrueckii were the predominant species of yeasts identifi edudin sourdoughs.udLactic acid bacteria and yeasts of liquid sourdoughs after fermentation were in the range of 9.61–9.89 log cfu/g and 6.55–7.36 log cfu/g, respectively.udVarious chemical parameters such as pH, total titratable acidity, lactic and acetic acids, ethanol and sugars were determined for liquid sourdoughs.udAcidifi cation and metabolite contents of these products were different, depending on the starter culture, fl our type and dough yield. Total titratableudacidity was more pronounced in the sourdoughs produced with whole wheat fl our (14.08 mL NaOH) and rye fl our (13.56 mL NaOH), dough yield 250ud(13.93 mL NaOH) and control sample (13.12 mL NaOH) which were produced without inoculum.