HIGH EXPRESSION RECOMBINANT VECTOR OF E. COLI AND PREPARING METHOD OF HUMAN GRANULOCYTE COLONY STIMULATING FACTOR

摘要

A gene of phosphorus granulocyte colony stimulating factor comprising the nucleotide sequence of the following formula (I).;(Wherein, W represents an upstream DNA sequence including the promoter and shine-dalgano region of the gene of the phosphorus granulocyte colony stimulator as described above, wherein the shinedalgano region is defined between the shine-dalgano region and the initiation codon. A DNA base sequence of 7 to 11 bp comprising a base sequence selected from the group consisting of formulas (e) to (g),;The promoter region is a trp promoter; X represents the 5 'terminal DNA sequence of the gene of the above-mentioned phosphorus granulocyte colony stimulator selected from the group consisting of the following formulas (a) to (d);;Y represents a DNA sequence corresponding to the 11 to 174 amino acid sequence of the above phosphorus granulocyte colony stimulator gene; Z is a DNA sequence containing a transcription terminator below the end codon of the phosphorus granulocyte colony stimulator gene, which is a transcription terminator of a tetracycline resistance gene, a transcription terminator of bacteriophage fd, a transcription terminator of Staphylococcus protein A, transforming the host cell using a recombinant vector comprising a rrnB operon transcription terminator) can overexpress hG-CSF in the host cell.