HIGHLY SENSITIVE DETERMINATION OF D-3-HYDROXYBUTIC ACID OR ACETIC ACID ACID AND COMPOSITION THEREFOR

摘要

Determination of D-3-hydroxybutyric acid or acetoacetic acid by reacting a specimen solution with a reagent containing (1) D-3-hydroxybutyric acid dehydrogenase, (2) A1, and (3) B1 to form the cycle (I) and determining the amount of A2 formed or the amount of B1 consumed; and a composition containing the ingredients (1), (2), and (3) for use in the above determination method. The method and the composition make it possible to determine a small amount of a specimen solution conveniently with high sensitivity and are useful in the field of clinical inspection and food testing. CHEM