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Measuring level of protein biosynthesis using photoprotein loaded with co-factor during translation as reporter for screening for biologically active compounds
Measuring level of protein biosynthesis using photoprotein loaded with co-factor during translation as reporter for screening for biologically active compounds
A method for monitoring the level of protein biosynthesis is new and comprises using a DNA or RNA template (I) encoding a photoprotein (II), as reporter, which is expressed in a protein biosynthesis system. (II) becomes loaded with a co-factor (III) during its translation and bioluminescence of the loaded (II) is measured.
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