Test strip for immersion for nucleic acid -, hybridization assays and method for covalent immobilization of oligonucleotides

摘要

Novel methods and compositions for detecting a member of a ligand pair on solid supports having intrinsic fluorescence are disclosed. A target member of a ligand pair is contacted with a capture member of a ligand pair, wherein the capture member is immobilized on a solid support having intrinsic fluorescence, and the contacted pair is in association with a colorimetric reporter, then the solid support is irradiated the solid support, and the resultant fluorescence is determined. Preferably, methods include hybridization assays of nucleic acid sequences on such solid supports. The extent of detecting a member of a ligand pair, preferably a nucleic acid sequence, is determined using a method or technique described throughout this document as fluorescent quenching. Methods and compositions pertaining to solid supports having their intrinsic or natural fluorescence quenched or masked are also described herein. The present invention has utility in detection assays for a member of a ligand pair. Such ligand pairs include, but are not limited to, antigens (or epitopes) and appropriate antibodies, complementary nucleic acid sequences, hormones and their receptors, enzymes and corresponding inhibitors, lectins and sugars, etc.