procedures for the development of new plant types with capacity to nitrogen in the leaves

摘要

PCT No. PCT/HU93/00020 Sec. 371 Date Nov. 3, 1994 Sec. 102(e) Date Nov. 3, 1994 PCT Filed Apr. 9, 1993 PCT Pub. No. WO93/20685 PCT Pub. Date Oct. 28, 1993The invention relates to a process for developing plants of novel type being capable of nitrogen-fixation also in their leaves. The process of the invention comprises inoculating plant protoplasts, cells, tissues, embryos or organs grown and/or treated under in vitro conditions with bacteria belonging to the family of Azotobacteraceae, then cocultivating the thus-obtained culture at a temperature of 15 DEG to 35 DEG C. and, if desired, propagating and/or regenerating the whole plant under in vitro conditions on or in a culture medium containing nitrogen and main carbon source(s) utilizable only by the plant cells as well as optionally other additives. The process of the invention ensures a well-balanced growth of the plant together with the bacteria.