首页>
外国专利>
DNS POLYMERASE WITH HIGHER TEMPERATURE STABILITY AND GROWTH AND EFFICIENCY OF PRIMER TRANSLATION
DNS POLYMERASE WITH HIGHER TEMPERATURE STABILITY AND GROWTH AND EFFICIENCY OF PRIMER TRANSLATION
展开▼
机译:具有较高温度稳定性和生长性的DNA聚合酶,并且具有较高的引物翻译效率
展开▼
页面导航
摘要
著录项
相似文献
摘要
A DNA polymerase having an amino acid sequence comprising substantially the same amino acid sequence as that of Thermus aquaticus or Thermus flavus DNA polymerase, excluding the N-terminal 280 amino acid residues of Thermus aquaticus DNA polymerase or the N-terminal 279 amino acid residues of Thermus flavus DNA polymerase, recombinant DNA sequences encoding said DNA polymerases, vectors comprising said DNA sequences, and host cells containing such vectors. A formulation of thermostable DNA polymerases comprising a majority component comprised of at least one thermostable DNA polymerase of the type described above, wherein the DNA polymerase lacks 3'-exonuclease activity, and a minority component comprised of at least one thermostable DNA polymerase exhibiting 3'-exonuclease activity, and an improved method for amplifying nucleic acid sequences by polymerase chain reaction wherein the above formulation is mixed and used to catalyze primer extension, are also provided.
展开▼