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Method for eliciting an immune response using a gene expression system that co-delivers an RNA polymerase with DNA
Method for eliciting an immune response using a gene expression system that co-delivers an RNA polymerase with DNA
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机译:使用与DNA共同递送RNA聚合酶的基因表达系统引发免疫应答的方法
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摘要
The present invention relates to a gene expression system in eukaryotic cells. In particular, it relates to a self-initiating and self- sustaining gene expression system utilizing an RNA polymerase prebound to a DNA construct before the introduction of the complex into cells. This system is capable of functioning in the cell cytoplasm without integration into the genome of host cells, and does not require host cell factors for initiation and maintenance of gene expression. Therefore, the invention has a wide range of applications in in vitro and in vivo gene expression, including gene therapy in resting cells.
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