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Cotton somaclonal variants

机译:棉花体细胞无性变异

摘要

A somaclonal variant cotton plant. The somaclonal cotton plant is produced by a method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.
机译:体细胞无性的棉花植物。体细胞无性系棉花植物通过以下方法生产:提供棉花外植体,在补充有葡萄糖作为主要碳源的愈伤组织生长培养基中培养该外植体,直到酚类化合物的分泌停止并且从外植体形成未分化的愈伤组织,再培养未分化的植物。补充蔗糖作为主要碳源的愈伤组织生长培养基中的愈伤组织,直到未分化的愈伤组织形成胚性愈伤组织,将胚性愈伤组织转移到植物发芽培养基中,在植物发芽培养基上培养胚性愈伤组织,直到从胚芽形成愈伤组织愈伤组织,将小植株转移到土壤中,使小植株生长以通过自花授粉产生种子,收集种子,播种种子,在选择所需特性的条件下生长种子并收集具有所需特性的植物。

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