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Method for identifying weeds resistant to inhibitors of acetolactate synthase

机译:鉴定对乙酰乳酸合酶抑制剂有抗性的杂草的方法

摘要

Resistance of given plant tissue to an inhibitor of acetolactate synthase is identified byPP(a) combining in an aqueous medium a sample of the plant tissue, the inhibitor of acetolactate synthase, and an inhibitor of keto acid reductoisomerase; P P(b) allowing time for acetolactate to accumulate;P P (c) rupturing the cells;PP(d) acidifying the mixture to convert any accumulated acetolactate to acetoin; andP P(e) calorimetrically detecting the presence of acetoin in the mixture.
机译:通过以下方式鉴定给定植物组织对乙酰乳酸合酶抑制剂的抗性:(a)在水性介质中结合植物组织的样品,乙酰乳酸合酶抑制剂和酮酸还原异构酶抑制剂; P>(b)留出时间使乙酰乳酸积聚;

(c)破坏细胞;

(d)酸化混合物以将任何积聚的乙酰乳酸转化为乙酰丁酸酯;和

(e)量热法检测混合物中乙酰丙酮的存在。

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