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Insecticidal factor from field peas

机译:豌豆的杀虫因子

摘要

A method and composition is provided to control insect pests using pea extracts. Pea extracts, protein-rich, fibre-rich and starch- rich fractions were tested for toxicity against various grain storage pests. The pea protein-rich extract was found to be toxic against the lesser grain borer, red flour beetle, rice weevil, maize weevil, granary weevil and the rusty grain beetle. The pea protein-rich extract also reduced the number of offspring of all insects tested. The protein-rich fraction was also found to be effective against the bertha army worm, diamondback, grasshopper, Indian meal moth and flea beetle. A partially purified active extract from the protein-rich fraction has been identified, using ion-exchange chromatography, reversed-phase chromatography, adsorption chromatography and gel filtration. However, the active ingredient within this fraction is not a protein with a molecular weight above 4,000 daltons, nor is it a protease inhibitor, lectin or soyasaponin I.
机译:提供了使用豌豆提取物防治害虫的方法和组合物。测试了豌豆提取物,富含蛋白质,富含纤维和富含淀粉的馏分对各种谷物储藏害虫的毒性。发现富含豌豆蛋白的提取物对小against虫,红色面粉甲虫,稻象鼻虫,玉米象鼻虫,粮谷象鼻虫和生锈的谷类甲虫有毒。富含豌豆蛋白的提取物还减少了所有测试昆虫的后代数量。还发现富含蛋白质的部分可有效抵抗伯莎氏菌,小菜蛾,蚱hopper,印度粉蛾和跳蚤甲虫。使用离子交换色谱法,反相色谱法,吸附色谱法和凝胶过滤法已鉴定出了富含蛋白质的馏分的部分纯化的活性提取物。但是,该级分中的活性成分不是分子量> 4,000道尔顿以上的蛋白质,也不是蛋白酶抑制剂,凝集素或大豆皂苷I。

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