A mutant of mono-oxygenase cytochrome P-450cam in which cysteine, at position 334, is deleted therefrom, or replaced by a different amino acid, is described. Tyrosine(96) may also be substituted by a different amino acid. The amino acid may be selected from alanine, arginine, asparagine, aspartic acid, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, proline, serine, threonine, tryptophan and valine, and, for cysteine(334), tyrosine. Preferred substitutions of amino acids may also occur at at least one of the positions 87, 98, 101, 185, 193, 244, 247, 295, 297, 395 and 396.
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