FORMATE DEHYDROGENASE GENE, RECOMBINANT VECTOR CONTAINING THE SAME, TRANSFORMANT CONTAINING THE RECOMBINANT VECTOR AND PRODUCTION OF FORMATE DEHYDROGENASE USING THE TRANSFORMANT

摘要

PROBLEM TO BE SOLVED: To obtain a new formate dehydrogenase gene which encodes a formate dehydrogenase containing a specific amino acid sequence and is useful in the inexpensive production or the like of the above enzyme that possesses high specific activity, shows a small Km value to formic acid and NAD+ and exerts the broad temperature or pH stability. ;SOLUTION: This formate dehydrogenase gene is a new gene which encodes a formate dehydrogenase comprising of the amino acid sequence represented by the formula or the amino acid sequence in which one or plural amino acids are deleted, substituted or added to the amino sequence represented by the formula and is useful for producing the formate dehydrogenase that possesses high specific activity, shows a small Km value to formic acid and nicotinamide adenine dinucleotide (NAD+) and exerts the broad temperature or pH stability conveniently in a large quantity and at a low cost. This gene is obtained by separating a chromosomal DNA from the genus Hyphomicrobium strain JT-17 (FERM P-16973) by a conventional method, preparing a chromosomal DNA library by the use of this chromosomal DNA and then screening out this library with a probe comprising the partial sequence.;COPYRIGHT: (C)2000,JPO