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METHOD FOR DIFFERENTIATION OF IMMATURE HEPATOCYTE TO MATURE HEPATOCYTE
METHOD FOR DIFFERENTIATION OF IMMATURE HEPATOCYTE TO MATURE HEPATOCYTE
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机译:不成熟肝细胞分化为成熟肝细胞的方法
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摘要
PROBLEM TO BE SOLVED: To differentiate immature hepatocytes to mature hepatocytes through a method for screening a compound controlling the differentiation of an immature hepatocyte or the like, which comprises culturing the immature hepatocyte in the presence of oncostatin M and dexamethasone. ;SOLUTION: Immature hepatocytes are differentiated to mature hepatocytes as follows: an embryo hepatic tissue of a mouse or the like is finely minced; the minced tissue is dispersed in a dispersing buffer containing an enzyme; subsequently blood cells are removed by the hemolysis with a hypotonic buffer solution; the obtained matter is suspended in a medium; it is plated on a tissue culture dish coated with gelatin; after several hours, hematopoietic cells and cell fragments are removed by washing with the medium to obtain immature hepatocytes; subsequently, the immature hepatocytes are culture in the presence of oncostatin M and dexamethasone; and the differentiation of the immature hepatocytes to mature hepatocytes is detected by using cell cluster formation, intracellular glycogen accumulation, intracellular albumin production, TAT gene expression, G6P-ase gene expression, ammonia detoxification or the like as an index.;COPYRIGHT: (C)2000,JPO
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