PURPOSE:To obtain the title gene containing a structural gene having a new specific base sequence and promoter region thereof, usable for degradation and decomposition of lignin in production of pulp and suitable for producing industrially useful lignin peroxidase. CONSTITUTION:Bjerkandern adusta is subjected to shake culture in glucose peptone culture at 28 deg.C for 7hr and the fungi cell is collected and frozen in a liquid nitrogen, powdered and centrifuged to remove fungi cell residue and a protein is removed from the supernatant and isopropanol is added to the supernatant to precipitate and separate a DNA and the DNA is subjected to treatment with restriction enzyme and bound to a vector and infected with Escherichia coli by subjecting the DNA to packaging to afford chromosome DNA library, which is then cultured in a culture medium and the produced colony is screened with a probe to select a colony containing DNA coding lignin peroxidase and plasmid DNA is extracted from the selected colony using an alkali extraction method and subjected to treatment with restriction enzyme and then lignin peroxidase gene is detected by Southern hybridization method and cut out from the medium to provide the objective gene.
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