LAK ACTIVITY POTENTIATOR ARISING FROM EXTRACT FROM MYCELIA OF LENTINUS EDODES

摘要

PROBLEM TO BE SOLVED: To obtain a LAK activity potentiator useful as an antitumor agent or anticancer agent, particularly an immunotherapeutic agent used in the LAK (lymphokine activated killer) therapy, which has no adverse drug reactions and is inexpensively available by including an extract from mycelia of Lentinus edodes. SOLUTION: This LAK activity potentiator is obtained by including an extract from mycelia of Lentinus edodes. The above extract may be obtained by initially multiplying Lentinus edodes fungi on a solid culture medium containing strained lees of Saccharum officinarum L. and nonfat rice bran as base materials, loosening the solid culture medium containing the resultant mycelia followed by crushing it to pieces at 30-35 deg.C in the presence of water and an enzyme selected from cellulase, protease or glucosidase to decompose, then heating it at 95 deg.C or lower to not only deactivate the enzyme but also sterilize and finally filtrating the resultant suspension. The above extract is preferably treated aseptically with acetone before adding, though the extract can directly be added into peripheral blood. When the above extract is directly added, it is favorable that the concentration is 10-50 g/ml.