首页> 外国专利> PRODUCTION OF BIOCHEMICALLY ACTIVE PEPTIDE BY EXPRESSION OF MODIFIED STORAGE SEED PROTEIN GENE IN CONGENIC PLANT

PRODUCTION OF BIOCHEMICALLY ACTIVE PEPTIDE BY EXPRESSION OF MODIFIED STORAGE SEED PROTEIN GENE IN CONGENIC PLANT

机译:在转基因植物中表达改良贮藏种子蛋白基因生产生物化学活性肽。

摘要

PROBLEM TO BE SOLVED: To obtain a new DNA fragment for production or the like of useful polypeptides by congenic plants which contain the seed peculiar promoter domain of Arabidopsis thaliana gene coding a 2S albumin precursor. SOLUTION: This DNA fragment is a new DNA fragment, which is an isolated DNA fragment containing the seed peculiar promoter domain of Arabidopsis thaliana gene coding the 2S albumin precursor and contains the amino acid sequence selected from the group consisting of AT2S1, AT2S2, AT 2S3 and AT2S4 of the formula in this 2S albumin, and is useful in the method or the like of forming useful and biologically active polypeptides by modified suitable congenic plants. This DNA fragment is obtained by screening out a genome library prepared by using the genome DNA in Arabidopsis thaliana with an oligonucleotide probe containing the partial sequence.
机译:解决的问题:通过含有编码2S白蛋白前体的拟南芥基因的种子独特启动子结构域的同基因植物获得用于生产有用多肽的新DNA片段或类似物。解决方案:该DNA片段是一个新的DNA片段,它是一个分离的DNA片段,包含编码2S白蛋白前体的拟南芥基因的种子独特启动子结构域,并包含选自AT2S1,AT2S2,AT 2S3的氨基酸序列在该2S清蛋白中具有式(I)和式(2)的AT2S4,并且在通过修饰的合适的同基因植物形成有用的生物活性多肽的方法等中有用。通过用含有部分序列的寡核苷酸探针筛选拟南芥中的基因组DNA而制备的基因组文库,从而获得该DNA片段。

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