16S RIBOSOMAL-RNA GENE, AND DETECTION OF PS. ALCALIGENES KB2 STRAIN BY USING DNA SEQUENCE IN THE GENE

摘要

PROBLEM TO BE SOLVED: To obtain a new 16S rDNA gene of Pseudomonas alcaligenes KB2 strain having a specific base sequence, and usable for rapid, simple, specific and highly sensitive detection or the like of this microorganism useful for environmental clean-up or the like. ;SOLUTION: This gene is a new 16S rDNA gene of Pseudomonas alcaligenes KB2 strain, and is used for rapid, simple, specific and highly sensitive detection or the like of the Pseudomonas alcaligenes KB2 strain capable of degrading a hardly degradable compound such as an aromatic hydrocarbon and an organic chlorine-based compound causing soil pollution. The gene is obtained by constructing a DNA library by using 16S rRNA prepared by a conventional method from the Pseudomonas alcaligenes KB2 strain, and screening the library by using a partial fragment of the 16S rRNA gene of a known Gram-negative aerobic bacterium as a probe.;COPYRIGHT: (C)2000,JPO