HIGH SENSITIVE IMMUNOLOGICAL DETECTION MEASURING METHOD FOR MICROORGANISM (BACTERIA, FUNGUS, VIRUS, PRODUCING SUBSTANCE) AND QUANTITATIVE METHOD

摘要

PROBLEM TO BE SOLVED: To quantitatively detect a microorganism at a high sensitivity by producing a product by adding dropwise an enzyme substrate to a microorganism dual antibody binding substance remained on a solid phase matrix. ;SOLUTION: A solid phase matrix requires a pore diameter through which a microorganism can not be passed. For example, the pore diameter is particularly preferably 0.22-0.45 μm in a bacteria inspection and 0.80 μm in the case of a fungus. After the microorganism is filtered and accumulated on the solid phase matrix if necessary, a sample solution remained on the solid phase matrix is washed with a washing liquid. A specific antibody to be detected is added dropwise to the microorganism on the solid phase matrix and a reaction of the microorganism with the antibody is carried out. As the antibody, an antibody against a specific microorganism is sometimes used if desired. A substance necessary for an enzymatic reaction is added dropwise to an enzyme labeled antibody binding microorganism captured and remained on the solid phase matrix and an enzymatic reaction is carried out. A product is finally produced and is measured. A determination of the desired microorganism is carried out by previously preparing a calibration line representing a reaction amount in the case of using a constant amount of antigen and applying it thereto.;COPYRIGHT: (C)2000,JPO