Procedure for the purification of recombinant human Erythropoietin from Culture supernatants of cells and Erythropoietin humanarecombinante obtained by such process

摘要

Refers to a method for the production of recombinant human EPO with high degree of purity.The process claimed also includesAvoid Exposing the Protein to extreme conditions of temperature and Organic Solvents that pudierana without compromising its biological activity or be toxic for human use.The Procedure involves a differential precipitation Chromatography, Hydrophobic Interaction Chromatography, concentration ydiafiltracion, sucesivaanion ica and cationicNew and diafiltracion concentration and molecular Exclusion Chromatography.The Procedure hasbeen designed by not using high pressure Liquid Chromatography steps and a high percentage of recovery of EPO.Includes also the epoobtenida according the procedure described.