Earlier stages of proteases, which are autocatiliticamente capitalized and its use

摘要

A process for the preparation of recombinant microbial protease, characterized by: (a) transforming a host cell with a recombinant Nucleic AcidWhich encodes an inactive Stage after a proteasamicr obiana,Which is identified by the active form of laproteasa mentioned and is cleaved to give especu00edfciamente protease Active; (b) cultivation of the host Cell so that the prior stage of proteinSegenera asa in the host cell in the form of inclusion bodies; (c) isolation of inclusion bodies and Naturalization under conditions in which the protease of Stage planIt generates in its natural conformation; and (d) of autocatalytic cleavage Stage prior laproteasa naturalized in active.