Genetic transformation of trees by introduction of disarmed Agrobacterium cells

摘要

he application is an improvement in the transformation of trees and provides a method which is less stressful to the tree being transformed. Notoriously difficult trees such as Eucalyptus globulus can be successfully transformed. An incision is made into a site capable of shoot initiation, disarmed Agrobacterium cells carrying one or more exogenous DNA sequences are introduced into the incision and the site is subjected to a selection step for a time period sufficient to produce transformed shoots.