Recombinant (humanised) ribonuclease proteins derived from a native ribonuclease found in the oocytes of Rana pipiens and uses therefor

摘要

The invention relates to ribonucleases derived from a native ribonuclease found in the oocytes of Rana pipiens. Various humanized and recombinant forms of these molecules are described as well as uses for them. The inventors had constructed recombinant ribonucleases that are highly cytotoxic after modification of the native ribonuclease. The inclusion of portions of mammalian or human-derived ribonucleases such as angiogenin or human eosinophil derived neurotoxin seems to be useful in the treatment of tumours sensitive to cytotoxic reagents.