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Immunohistochemical assay for carcinoma proliferative status by detecting presence of NGAL protein
Immunohistochemical assay for carcinoma proliferative status by detecting presence of NGAL protein
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机译:通过检测NGAL蛋白的存在来进行免疫组化分析以检测癌症的增殖状态
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摘要
An immunohistochemical staining method for determining the relative degree of proliferation of a human breast carcinoma, comprising: 1. obtaining a sample of the carcinoma with the surrounding tissue; 2. using an antibody having specificity for NGAL protein to bind to NGAL protein in the sample; and 3. using a marker bound to the antibody to indicate the relative extent to which NGAL protein is present in the carcinoma as compared to the surrounding tissue. The amount of NGAL expression product is compared with a standard curve to determine the S-phase value. The sample can be breast tissue or breast fluid aspirate. Alternatively, blood can be analyzed for this marker to diagnose metastasis.
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