PROCEDURE FOR THE SIMULTANEOUS QUANTIFICATION IN A SINGLE MEDICINE OF THE MAIN TYPES OF HUMAN LYMPHOCYTES AND ITS SUB-ASSUMPTIONS

摘要

Procedure for the simultaneous quantification, in a single measurement, of the major types of human lymphocytes and their subsets. It includes: incubation of the sample with five different monoclonal antibodies conjugated with three different fluorochromes; measurement by flow cytometry of the three fluorescent emissions; and analysis of the results obtained by means of multiparametric analysis to determine the presence/absence of a certain fluorescence in a cell and its intensity in each cell.