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The induction of stress-related factors in plants.

机译:植物中与胁迫相关的因子的诱导。

摘要

The present invention relates to a method of producing resistance to stress in a variety of plants by the expression of a hydrogen peroxide or reactive oxygen species producing enzyme such as, but not limited to, oxalate oxidase. In addition, the present invention relates to a method of producing a stress resistant sunflower plant by transforming a sunflower plant with an oxalate oxidase gene or other hydrogen peroxide/reactive oxygen species producing genes that upon expression of the gene produce an enzyme capable of generating hydrogen peroxide or reactive oxygen species from endogeously available substrate. Alternatively, the plant could be transformed with the hydrogen peroxide or reactive oxygen species producing gene and a gene for the necessary substrate or a gene which would modify a pathway in the plant resulting in formation or increase in substrate. The production of hydrogen peroxide or a reactive oxygen species triggers the expression of hydrogen peroxide/reactive oxygen species-responsive genes resulting ina pre-challenge accumulation of pathogenesis or stress-related factors such as salicylic acid and PR protein. Thus the plant is rendered resistant to a variety of stresses by, for example, inhibiting the establishment of a pathogen resulting in a disease resistance phenotype.
机译:本发明涉及通过表达过氧化氢或产生活性氧的酶例如但不限于草酸氧化酶在多种植物中产生抗逆性的方法。另外,本发明涉及通过用草酸盐氧化酶基因或其他过氧化氢/活性氧物种产生基因转化向日葵植物而产生抗逆性向日葵植物的方法,所述基因在该基因表达后产生能够产生氢的酶。来自内生基质的过氧化物或活性氧。或者,可以用产生过氧化氢或活性氧物种的基因和必需底物的基因或将修饰植物中的途径从而导致底物形成或增加的基因来转化植物。过氧化氢或活性氧物种的产生触发了过氧化氢/活性氧物种响应性基因的表达,导致发病前或与压力相关的因素(例如水杨酸和PR蛋白)的挑战前累积。因此,例如通过抑制导致疾病抗性表型的病原体的建立,使植物对多种胁迫具有抗性。

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