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Rapid tests for the assessment of state organs based on the detection of one or more isoenzymes of glutathione-s transferase.

机译:基于一种或多种谷胱甘肽转移酶同工酶的检测,快速评估状态器官。

摘要

SE describes a method for rapid assessment STATE OF ORGANS, INCLUDING INJURIES organs after an immunological or toxicological ATTACK ON A SUBJECT BASED ON THE DETECTION OF ONE OR MORE ISOENZYMES Glutathione S ferasa (GST) IN DIFFERENT BIOLOGICAL FLUIDS THAT includes contacting an anti specific to that isoenzyme with a sample of a biological fluid WHICH IS SUSPECTED containing said isoenzyme, said aNTIBODY A sufficiently sensitive to detect at least one picomolar amount of said isoenzyme, AND CAPTURE THE COMPLEX isoenzyme FILM ON A immobilized capture antibody, to generate a signal detectable visually. PROCEDURE allows detection or determination of GST isoenzymes in a typical order of 30 minutes time from initial contact with the anti LLO TO USE AND THEREFORE MAY BE USED, FOR EXAMPLE, BY PATIENTS AT HOME TO CONTROL LEVELS A ISOENZIMAGST CONCRETA after a transplant liver or kidney.
机译:SE描述了一种快速评估器官状态的方法,其中包括基于检测不同生物流体中的一种或多种同工酶谷胱甘肽S(SST)(GST)的方法,对受试者进行免疫学或毒理学攻击后伤害器官,包括接触与该物质相关的特异性抗含有可能含有上述同工酶的生物液体样品中检测到的同工酶,所述aNTIBODY A足够灵敏以检测至少一个皮摩尔量的同工酶,并在固定的捕获抗体上捕获复杂的同工酶膜,以产生视觉上可检测的信号。该程序允许从与抗LLO初次接触开始到使用后通常在30分钟内检测或确定GST同工酶,因此可以使用这种方法,例如,由在家中的患者控制移植肝或肾后的同工酶凝结。

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