MOUSE MONOCLONAL ANTIBODIES TO HIV-1P24 AND THEIR USE IN DIAGNOSTIC TESTS

摘要

The present invention provides monoclonal antibodies demonstrating specificreactivitywith HIV-I p24. One monoclonal antibody designated 31-42-19 recognizes anunique epitopeon HIV-I p24 that is not immunogenic in humans. 31-42-19 also reacts with anantigenicallycross reactive epitope on HIV-2 p24. Another monoclonal antibody designated 31-90-25recognizes an epitope within a highly immunogenic region of HIV-I p24. Thepresent inventionalso provides cell lines capable of producing these monoclonal antibodies. Theinvention alsoincludes a highly sensitive enzyme immunoassay for the detection of HIV-I p24in biologicalfluids, using a monoclonal antibody mixture. The present invention furtherprovides methodsfor the use of these monoclonal antibodies for the detection of anti-HIV-I p24antibodies andHIV-2 p24 antigen in biological samples.