ORNITHINE CARBAMOYL TRANSFERASE GENE AND UTILIZATION OF THE DNA

摘要

This invention relates to a novel ornithine carbamoyltransferase (OCTase) gene, a recombinant DNA containing theOCTase DNA, a transformation system for a basidiomycete,and production of useful proteins using the system.More particularly, this invention relates to the OCTasegene of Coriolus hirsutus to afford an efficient host-vectorsystem in basidiomycetes (particularly a white rot fungussuch as Coriolus hirsutus IFO 4917) for the preparation ofuseful proteins. OCTase is the enzyme to transformornithine to citrulline in arginine biosynthesis inorganisms. The present invention provides the OCTase genesof C. hirsutus, the useful Arg~ auxotrophic mutant of C.hirsutus deficient in the OCTase gene, the efficientcondition for the preparation of protaplasts and thetransformation of the mutant with the cloned OCTase gene andthe recombinant DNA's including a promoter, a signalpeptide-coding DNA and a protein-coding DNA, theconstruction of the navel host-vector system of C. hirsutus,and the new method of a large scale preparation of usefulproteins using this novel host-vector system with a newrecombinant DNA technique.Furthermore, this invention provides a highly efficientmethod to produce a useful protein such as lignin peroxidasewhich is difficult to produce by the conventional method.