METHOD FOR IDENTIFYING AND REPRESENTING INTERINDIVIDUAL DIFFERENCES OF DNA SEQUENCES

摘要

The invention relates to a method for identifying and representinginterindividual differences of DNA sequences. The invention is characterizedin that; the genomic DNA of the individuals to be examined are isolated;segments of the genes to be compared, genomic regions, and genomes aredefined; these segments are isolated in a targeted manner by means of PCRwhile using highly specific oligonucleotides and are produced in a sufficientnumber of copies; a simultaneous sequencing of all obtained gene segments iscarried out, said gene segments now being available as PCR products;afterwards, the mixture of termination reactions which is produced thereby andwhich contains the individual sequences of all gene segments iselectrophoretically separated on a sequencing gel; the mixture is transferredonto a membrane by means of direct transfer electrophoresis; afterwards, theindividual sequences of a determined gene segment from the original mixtureare made visible by hybridizing the membrane with a marked, highly specificoligonucleotide; this process is repeated unit all gene segments from thecommon pool are detected; afterwards, interindividual differences of DNAsequences are detected by image analysis, and a genetic profile model isestablished and evaluated by mathematical processing.