NON-HUMAN TRANSGENIC ANIMAL WHOSE GERM CELLS AND SOMATIC CELLS CONTAIN A KNOCKOUT MUTATION IN DNA ENCODING ORPHAN NUCLEAR RECEPTOR ERRALPHA

摘要

The present invention relates to a transgenic non-human mammal whose germcells and somatic cells contain a knockout mutation in DNA encoding orphannuclear receptor ERR.alpha.. More specifically, the invention relates toknockout mice whose germ cells and somatic cells contain a knockout mutationin DNA encoding orphan nuclear receptor ERR.alpha.. The invention furtherrelates to such knockout non-human mammals which express an ERR.alpha. genewhich is different from the endogenous gene which was disrupted. In aparticular embodiment, the invention relates to a transgenic mouse having itsendogenous ERR.alpha. gene disrupted and expressing human ERR.alpha.. As well,the invention relates to cell lines in which ERR.alpha. activity (and/orlevel) has been inactivated or augmented. The invention further relates touses and methods of the transgenic animals of the present invention to selectagents which modulate the expression and/or activity of ERR.alpha., inparticular in relation to fatty acid metabolism.