A purification process for a calcium binding type enzyme, such as a metalloprotease, using a calcium free buffer

摘要

The present invention relates to an enzyme purification process. More particularly, the present invention relates to a protein purification process for calcium binding type enzyme. Specifically, in one aspect of the present invention there is provided a process for purifying a calcium binding type enzyme which is a mettalloprotease comprising: (a) preparing an unpurified solution which contains calcium binding type enzyme; (b) concentrating the solution; (c) diluting by adding calcium free buffer; (d) adjusting buffer concentration and pH, (e) incubating the concentrated solution to precipitate the protein; and (f) collecting the precipitate.